Wear gloves and safety glasses when handling gels The separating range of Tricine gels is 2.5-200 kDa. Tricine gels must be used with denatured or reduced proteins only. The Novex® Tricine Gels do not contain tricine in the gel, the tricine is supplied by the running buffer. In this buffer system, tricine substitutes glycine in the running buffer resulting in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides. The Tricine Gels are based on the Tricine system developed by (Schaegger and vonJagow, 1987). Novex® Tricine Gels are ideal for peptides and low molecular weight proteins (less than 10 kDa). The smaller proteins that migrate with the stacked DS micelles in the Tris-Glycine system are now well separated from DS ions in the Tricine system resulting in sharper bands and higher resolution. To solve this problem, the Tricine system uses a low pH of the gel buffer and replaces the trailing glycine ion with a fast moving tricine ion in the running buffer. The mixing also interferes with the fixing and staining of smaller proteins. This zone of stacked DS micelles causes mixing of the DS ions with the smaller proteins resulting in fuzzy bands and decreased resolution.
However, the resolution of smaller proteins (<10 kDa) is hindered by the continuous accumulation of free dodecylsulfate (DS) ions (from the SDS sample and running buffers) in the stacking gel. These stacked protein bands undergo sieving once they reach the separating gel. In the Tris-Glycine system, the proteins are stacked in the stacking gel between a highly mobile leading chloride ion (in the gel buffer) and the slower trailing glycine ion (in the running buffer). The Tricine system is a modification of the Tris-Glycine discontinuous buffer system specifically designed for the resolution of low molecular weight proteins. Minimizes protein modification as the Tricine buffer system has a lower pH.
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